| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Mouse/Dog |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-CBX5/HP1-Alpha, biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Biotin |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 25kDa band observed in HeLa lysates (calculated MW of 22.2kDa according to NP_036249.1). See non-biotinylated parental product's datasheet for further QC data. Recommended conc |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
CBX5 |
| Gene ID: |
23468 |
| Uniprot ID: |
CBX5_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_036249.1 |
| Specificity: |
Reported variants represent identical protein: NP_036249.1, NP_001120793.1, NP_001120794.1 |
| Immunogen Sequence: |
NKRKSNFSNSADDIK. |
| Function | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR). Involved in the formation of kinetochore through interaction with the MIS12 complex subunit NSL1. Required for the formation of the inner centromere. |
| Protein Name | Chromobox Protein Homolog 5Antigen P25Heterochromatin Protein 1 Homolog AlphaHp1 Alpha |
| Database Links | Reactome: R-HSA-4551638Reactome: R-HSA-8953750Reactome: R-HSA-983231Reactome: R-HSA-9843940 |
| Cellular Localisation | NucleusChromosomeCentromereColocalizes With Hnrnpu In The NucleusComponent Of Centromeric And Pericentromeric HeterochromatinAssociates With Chromosomes During MitosisAssociates Specifically With Chromatin During Metaphase And AnaphaseLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Chromobox Protein Homolog 5 antibodyAnti-Antigen P25 antibodyAnti-Heterochromatin Protein 1 Homolog Alpha antibodyAnti-Hp1 Alpha antibodyAnti-CBX5 antibodyAnti-HP1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
