• STJ70789 (0. 03µg/ml) staining of A43N (A) and K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70789 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70789 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-CBX5/HP1-Alpha antibody (Internal) (STJ70789)

SKU:
STJ70789-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CBX5/HP1-Alpha (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.03-0.1µg/ml
IF-Strong expression of the protein seen in the nuclei of U2OS cells. 10µg/ml
FC-Flow cytometric analysis of HeLa cells. 10ug/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CBX5
Gene ID: 23468
Uniprot ID: CBX5_HUMAN
Immunogen Region: Internal
Accession Number: NP_036249.1; NP_001120793.1; NP_001120794.1
Specificity: Variants (NP_036249.1; NP_001120793.1; NP_001120794.1) encode the same protein.
Immunogen Sequence: NKRKSNFSNSADDIK
Function Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Protein Name Chromobox Protein Homolog 5
Antigen P25
Heterochromatin Protein 1 Homolog Alpha
Hp1 Alpha
Database Links Reactome: R-HSA-4551638
Reactome: R-HSA-8953750
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Chromosome
Centromere
Colocalizes With Hnrnpu In The Nucleus
Component Of Centromeric And Pericentromeric Heterochromatin
Associates With Chromosomes During Mitosis
Associates Specifically With Chromatin During Metaphase And Anaphase
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Chromobox Protein Homolog 5 antibody
Anti-Antigen P25 antibody
Anti-Heterochromatin Protein 1 Homolog Alpha antibody
Anti-Hp1 Alpha antibody
Anti-CBX5 antibody
Anti-HP1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance