• Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from HeLa, A549, and K562 cells, using CBX5 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using HP1 Alpha Polyclonal Antibody diluted at 1：1000

Anti-CBX5 antibody (41-90 aa) (STJ93588)

SKU:
STJ93588

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Chromobox protein homolog 5 (41-90 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBX5
Gene ID: 23468
Uniprot ID: CBX5_HUMAN
Immunogen Region: 41-90 aa
Specificity: HP1 Alpha Polyclonal Antibody detects endogenous levels of HP1 Alpha protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CBX5 at the amino acid range 41-90
Function Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Protein Name Chromobox Protein Homolog 5
Antigen P25
Heterochromatin Protein 1 Homolog Alpha
Hp1 Alpha
Database Links Reactome: R-HSA-4551638
Reactome: R-HSA-8953750
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Chromosome
Centromere
Colocalizes With Hnrnpu In The Nucleus
Component Of Centromeric And Pericentromeric Heterochromatin
Associates With Chromosomes During Mitosis
Associates Specifically With Chromatin During Metaphase And Anaphase
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Chromobox Protein Homolog 5 antibody
Anti-Antigen P25 antibody
Anti-Heterochromatin Protein 1 Homolog Alpha antibody
Anti-Hp1 Alpha antibody
Anti-CBX5 antibody
Anti-HP1A antibody

Information sourced from Uniprot.org

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