| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-E3 SUMO-protein ligase CBX4 (196-246 aa) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CBX4 |
| Gene ID: |
8535 |
| Uniprot ID: |
CBX4_HUMAN |
| Immunogen Region: |
196-246 aa |
| Specificity: |
This antibody detects endogenous levels of CBX4 at Human/Mouse |
| Immunogen: |
Synthesized peptide derived from the human CBX4 at the amino acid range 196-246 |
| Post Translational Modifications | Ubiquitinated. Ubiquitination regulates the function of the Polycomb group (PcG) multiprotein PRC1-like complex. Deubiquitinated by USP26. Phosphorylated on Thr-497 by HIPK2 upon DNA damage. This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK. |
| Function | E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3). Plays a role in the lineage differentiation of the germ layers in embryonic development. |
| Protein Name | E3 Sumo-Protein Ligase Cbx4Chromobox Protein Homolog 4Polycomb 2 HomologPc2Hpc2 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-3108214Reactome: R-HSA-3899300Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4655427Reactome: R-HSA-8939243Reactome: R-HSA-8943724 |
| Cellular Localisation | NucleusNucleus SpeckleLocalization To Nuclear Polycomb Bodies Is Required For Znf131 SumoylationLocalized In Distinct Foci On Chromatin |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Cbx4 antibodyAnti-Chromobox Protein Homolog 4 antibodyAnti-Polycomb 2 Homolog antibodyAnti-Pc2 antibodyAnti-Hpc2 antibodyAnti-CBX4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
