| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CBLB (925-942) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CBLB |
| Gene ID: |
868 |
| Uniprot ID: |
CBLB_HUMAN |
| Immunogen Region: |
925-942 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 925-942 of human CBLB (NP_001308717.1). |
| Immunogen Sequence: |
PEAALENVDAKIAKLMGE |
| Tissue Specificity | Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes (at protein level). |
| Post Translational Modifications | Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation. Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28.which promotes proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA. |
| Protein Name | E3 Ubiquitin-Protein Ligase Cbl-BCasitas B-Lineage Lymphoma Proto-Oncogene BRing Finger Protein 56Ring-Type E3 Ubiquitin Transferase Cbl-BSh3-Binding Protein Cbl-BSignal Transduction Protein Cbl-B |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmUpon Egf StimulationAssociates With Endocytic Vesicles |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Cbl-B antibodyAnti-Casitas B-Lineage Lymphoma Proto-Oncogene B antibodyAnti-Ring Finger Protein 56 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Cbl-B antibodyAnti-Sh3-Binding Protein Cbl-B antibodyAnti-Signal Transduction Protein Cbl-B antibodyAnti-CBLB antibodyAnti-RNF56 antibodyAnti-Nbla00127 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
