• Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using CBL Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using CBL Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of HeLa cells using Cbl Polyclonal Antibody
  • Immunofluorescence analysis of A549 cells, using CBL Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Cbl Polyclonal Antibody

Anti-CBL antibody (740-789 aa) (STJ92055)

SKU:
STJ92055

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase CBL (740-789 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBL
Gene ID: 867
Uniprot ID: CBL_HUMAN
Immunogen Region: 740-789 aa
Specificity: Cbl Polyclonal Antibody detects endogenous levels of Cbl protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CBL at the amino acid range 740-789
Function Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Ubiquitinates SPRY2. Ubiquitinates EGFR. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA.
Protein Name E3 Ubiquitin-Protein Ligase Cbl
Casitas B-Lineage Lymphoma Proto-Oncogene
Proto-Oncogene C-Cbl
Ring Finger Protein 55
Ring-Type E3 Ubiquitin Transferase Cbl
Signal Transduction Protein Cbl
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-1236382
Reactome: R-HSA-1295596
Reactome: R-HSA-1433559
Reactome: R-HSA-182971
Reactome: R-HSA-2173789
Reactome: R-HSA-5637810
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-6807004
Reactome: R-HSA-8849469
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8875360
Reactome: R-HSA-912631
Reactome: R-HSA-9680350
Reactome: R-HSA-9706369
Reactome: R-HSA-9706377
Cellular Localisation Cytoplasm
Cell Membrane
Cell Projection
Cilium
Golgi Apparatus
Colocalizes With Fgfr2 In Lipid Rafts At The Cell Membrane
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Cbl antibody
Anti-Casitas B-Lineage Lymphoma Proto-Oncogene antibody
Anti-Proto-Oncogene C-Cbl antibody
Anti-Ring Finger Protein 55 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Cbl antibody
Anti-Signal Transduction Protein Cbl antibody
Anti-CBL antibody
Anti-CBL2 antibody
Anti-RNF55 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance