• Anti-CAV2 at 1:1000 dilution; 50 µg lysate per lane of transfected HEK293 and 100 µg of HaCat cell line; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-CAV2 antibody- Caveolae Marker at 1:100 dilution in NHI:3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin
  • Immunofluorescence – anti-CAV2 antibody- Caveolae Marker at 1:100 dilution in NHI:3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin
  • Immunohistochemistry of human skin using anti-CAV2 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CAV2 antibody at 1:500/DAB detection.
  • Immunohistochemistry of mouse lung using anti-CAV2 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CAV2 antibody at 1:500/DAB detection.
  • Immunohistochemistry of human lymph node using anti-CAV2 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CAV2 antibody at 1:500/DAB detection.

Anti-CAV2 antibody (50aa N-Term) (STJ140020)

SKU:
STJ140020

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Caveolin-2 (50aa N-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:2000
IF 1:50-1:200
IHC-P 1:200-1:1000
IHC-F 1:200-1:1000
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: CAV2
Gene ID: 858
Uniprot ID: CAV2_HUMAN
Immunogen Region: 50aa N-Term
Accession Number: ENSG00000105971
Specificity: Detects a band of approximately 20 kDa by Western blot in HaCat cell lysate and 45 kDa CAV2-GFP transfected cell lysate. This Ab does not recognize CAV1.
Immunogen: Recombinant peptide derived from within residues 50 aa to the N-terminus of human CAV2 produced in E. coli.
Immunogen Sequence: MDDDSYSHHSGLEYADPEKF ADSDQDRDPHRLNSHLKLGF EDVIAEPVTTH
Tissue Specificity Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
Post Translational Modifications Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells. Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
Function May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
Protein Name Caveolin-2
Database Links Reactome: R-HSA-9009391
Cellular Localisation Nucleus
Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cell Membrane
Membrane
Caveola
Potential Hairpin-Like Structure In The Membrane
Membrane Protein Of Caveolae
Tyr-19-Phosphorylated Form Is Enriched At Sites Of Cell-Cell Contact And Is Translocated To The Nucleus In Complex With Mapk1 In Response To Insulin
Tyr-27-Phosphorylated Form Is Located Both In The Cytoplasm And Plasma Membrane
Cav1-Mediated Ser-23-Phosphorylated Form Locates To The Plasma Membrane
Ser-36-Phosphorylated Form Resides In Intracellular Compartments
Alternative Antibody Names Anti-Caveolin-2 antibody
Anti-CAV2 antibody

Information sourced from Uniprot.org

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