| Host: | Sheep |
| Applications: | IHC/WB |
| Reactivity: | Mouse/Rat/Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Sheep polyclonal antibody anti-CAV1 (Cytoplasmic Domain) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | Cav1 |
| Gene ID: | 12389 |
| Uniprot ID: | CAV1_MOUSE |
| Immunogen Region: | Cytoplasmic Domain |
| Specificity: | Specific for Caveolin-1. |
| Immunogen: | A synthetic peptide from the 1t cytoplasmic domain of human Caveolin-1 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse. |
| Post Translational Modifications | The N-terminus of both isoforms are blocked. Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress. Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation. Ubiquitinated by ZNRF1.leading to degradation and modulation of the TLR4-mediated immune response. |
| Function | May act as a scaffolding protein within caveolar membranes. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation. Binds 20(S)-hydroxycholesterol (20(S)-OHC). |
| Protein Name | Caveolin-1 |
| Database Links | Reactome: R-MMU-203615Reactome: -MMU-203641Reactome: -MMU-210991Reactome: -MMU-4641262Reactome: -MMU-5218920Reactome: -MMU-8980692Reactome: -MMU-9009391Reactome: -MMU-9013026Reactome: -MMU-9013106Reactome: -MMU-9013148Reactome: -MMU-9013149Reactome: -MMU-9013404Reactome: -MMU-9013405Reactome: -MMU-9013406Reactome: -MMU-9013407Reactome: -MMU-9013408Reactome: -MMU-9013423Reactome: -MMU-9035034Reactome: -MMU-9696264Reactome: -MMU-9696270Reactome: -MMU-9696273 |
| Cellular Localisation | Golgi Apparatus MembranePeripheral Membrane ProteinCell MembraneMembraneCaveolaMembrane RaftGolgi ApparatusTrans-Golgi NetworkColocalized With Dpp4 In Membrane RaftsPotential Hairpin-Like Structure In The MembraneMembrane Protein Of Caveolae |
| Alternative Antibody Names | Anti-Caveolin-1 antibodyAnti-Cav1 antibodyAnti-Cav antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance