• STJ73733 (1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73733 (1µg/ml) staining of Human Lung (A) and HCORC (B) and (0. 5ug/ml) HCBGLC (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73733 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73733 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Cathepsin B antibody (Internal) (STJ73733)

SKU:
STJ73733-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Cathepsin B (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.5-2µg/ml
IHC-5µg/ml
IF-Strong expression of the protein seen in the cytoplasm/vesicles of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CTSB
Gene ID: 1508
Uniprot ID: CATB_HUMAN
Immunogen Region: Internal
Accession Number: NP_001899.1
Specificity: Reported variants represent identical protein: NP_680091.1, NP_680090.1, NP_680092.1, NP_001899.1, NP_680093.1.
Immunogen Sequence: SDRICIHTNAHVSVE
Function Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.
Protein Name Cathepsin B
App Secretase
Apps
Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1679131
Reactome: R-HSA-2022090
Reactome: R-HSA-2132295
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Melanosome
Secreted
Extracellular Space
Apical Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Localizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells
Alternative Antibody Names Anti-Cathepsin B antibody
Anti-App Secretase antibody
Anti-Apps antibody
Anti-Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain antibody
Anti-CTSB antibody
Anti-CPSB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance