Anti-CASP3 antibody [31A1067] (STJA0005296)

SKU:
STJA0005296-100

£319.50
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Host: Mouse
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Caspase-3 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 31A1067
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Protein G Purified
Dilution Range: WB 1:250
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP3
Gene ID: 836
Uniprot ID: CASP3_HUMAN
Specificity: The antibody detects pro Caspase-3 at 32 kDa* and the large subunit of the cleaved form of Caspase-3 at 14-21 kDa. The antibody can be used for western blot, immunohistochemistry and immuno-electron microscopy.
Immunogen: Clone 31A1067 was developed against full-length recombinant human Caspase-3. The antibody recognizes an epitope in the large domain subunit (p17) of Caspase-3, and it will recognize pro Caspase-3 and the p17 cleavage fragment.
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Post Translational Modifications Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP3 processing, preventing CASP3 activation and ability to recognize and cleave substrates.
Function Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
Protein Name Caspase-3
Casp-3
Apopain
Cysteine Protease Cpp32
Cpp-32
Protein Yama
Srebp Cleavage Activity 1
Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12
Database Links Reactome: R-HSA-111459
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-111465
Reactome: R-HSA-111469
Reactome: R-HSA-140342
Reactome: R-HSA-1474228
Reactome: R-HSA-2028269
Reactome: R-HSA-205025
Reactome: R-HSA-211736
Reactome: R-HSA-264870
Reactome: R-HSA-351906
Reactome: R-HSA-418889
Reactome: R-HSA-449836
Reactome: R-HSA-5620971
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Caspase-3 antibody
Anti-Casp-3 antibody
Anti-Apopain antibody
Anti-Cysteine Protease Cpp32 antibody
Anti-Cpp-32 antibody
Anti-Protein Yama antibody
Anti-Srebp Cleavage Activity 1 antibody
Anti-Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 antibody
Anti-CASP3 antibody
Anti-CPP32 antibody

Information sourced from Uniprot.org

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