Anti-CASP14 antibody (110-190 aa) (STJ90022)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from Caspase-14 at the amino acid range 110-190
General Information
| Short Description | Rabbit polyclonal anti-Caspase-14 (110-190 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CASP14 |
| Gene ID | 23581 |
| Uniprot ID | CASPE_HUMAN |
| Immunogen | Synthesized peptide derived from Caspase-14 at the amino acid range 110-190 |
| Immunogen Region | 110-190 aa |
| Specificity | Caspase-14 Polyclonal Antibody detects endogenous levels of Caspase-14 protein. |
Additional Info
| Post Translational Modifications | Maturation by proteolytic processing appears to be a two-step process. The precursor is processed by KLK7 to yield the p20/p8 intermediate form which acts on the precursor to yield the p17/p10 mature form. Initially, cleavage between Ile-152 and Lys-153 has been proposed to yield the large and small subunits of the active enzyme. |
| Function | Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum. Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin. In vitro has a preference for the substrate WY-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF. Involved in processing of prosaposin in the epidermis. May be involved in retinal pigment epithelium cell barrier function. Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD. |
| Protein Name | Caspase-14Casp-14 Cleaved Into - Caspase-14 Subunit P17 - Mature Form - Caspase-14 Subunit P10 - Mature Form - Caspase-14 Subunit P20 - Intermediate Form - Caspase-14 Subunit P8 - Intermediate Form |
| Database Links | Reactome: R-HSA-6809371Reactome: R-HSA-9725554 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Caspase-14 antibodyAnti-Casp-14 Cleaved Into - Caspase-14 Subunit P17 - Mature Form - Caspase-14 Subunit P10 - Mature Form - Caspase-14 Subunit P20 - Intermediate Form - Caspase-14 Subunit P8 - Intermediate Form antibodyAnti-CASP14 antibody |
Information sourced from Uniprot.org