• Western blot analysis of extracts of 293T cells and 293T transfected with CAS9 protein, using CAS9 Rabbit polyclonal antibody (STJ117195) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-CAS9 antibody (450-730) (STJ117195)

SKU:
STJ117195

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 450-730
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 450-730 of streptococcus pyogenes m1 gas CAS9 (NP_269215.1).
Immunogen Sequence: YYVGPLARGNSRFAWMTRKS EETITPWNFEEVVDKGASAQ SFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKY VTEGMRKPAFLSGEQKKAIV DLLFKTNRKVTVKQLKEDYF KKIECFDSVEISGVEDRFNA SLGTYHDLLKIIKDKDFLDN EENEDILEDIVLTLTLFEDR EMIEERLKTYAHLFDDKVMK QLKRRRYTGWGRLSRKLING IRDKQSGKTILDFLKSDGF
Background Staphylococcus aureus Cas9 (SaCas9) is an RNA-guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its small size facilitates in vivo delivery for genome editing in various organisms.

Information sourced from Uniprot.org

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