| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Other |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4974RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: |
450-730 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 450-730 of streptococcus pyogenes m1 Cas9. (NP_269215.1). |
| Immunogen Sequence: |
YYVGPLARGNSRFAWMTRKS EETITPWNFEEVVDKGASAQ SFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKY VTEGMRKPAFLSGEQKKAIV DLLFKTNRKVTVKQLKEDYF KKIECFDSVEISGVEDRFNA SLGTYHDLLKIIKDKDFLDN EENEDILEDIVLTLTLFEDR EMIEERLKTYAHLFDDKVMK QLKRRRYTGWGRLSRKLING IRDKQSGKTILDFLKSDGF |
| Background | CRISPR-associated protein 9 (Cas9) is an RNA-guided DNA nuclease and is a component of the Streptococcus pyogenes CRISPR-antiviral immune system that provides adaptive immunity against extrachromosomal genetic material. The mechanism of CRISPR antiviral action involves three steps: (i) acquisition of foreign DNA by host bacteria; (ii) synthesis and maturation of CRISPR RNA (crRNA) , followed by formation of RNA-Cas nuclease-protein complex; and (iii) acquisition of foreign DNA by the host bacterium; The complex recognizes foreign DNA and undergoes directed interference by cleavage by Cas nuclease activity. The type II CRISPR/Cas antiviral immune system provides a powerful tool for precise genome editing and has the potential for specific regulation of genes and therapeutic applications. The Cas9 protein and guide RNA (comprising a fusion between crRNA and transactivating crRNA (tracrRNA) ) must be introduced or expressed in the cell. A 20-nucleotide sequence at the 5 end of the guide RNA guides Cas9 to a specific DNA target site. Thus, Cas9 can be "programmed" to cut a variety of DNA sites in vitro and in cells and organisms. The CRISPR/Cas9 genome editing tool has been used in many organisms, including mouse and human cells. Studies have shown that CRISPR can be used to generate mutant alleles or reporter genes in rodent and primate embryonic stem cells. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
