![Immunohistochemistry analysis of paraffin-embedded rat kidney using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/99260/384043/STJ22890_1__14526.1713152683.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded rat kidney using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of paraffin-embedded mouse liver using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/99260/384044/STJ22890_2__27798.1713152684.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded mouse liver using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of paraffin-embedded human colon using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/99260/384045/STJ22890_3__64106.1713152685.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded human colon using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of paraffin-embedded rat kidney using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/99260/384043/STJ22890_1__14526.1713152683.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded rat kidney using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:100-1:500 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CARM1 |
| Gene ID: | 10498 |
| Uniprot ID: | CARM1_HUMAN |
| Immunogen Region: | 486-603 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 486-603 of human PRMT4/CARM1 (NP_954592.1). |
| Immunogen Sequence: | GSHYTSPSENMWNTGSTYNL SSGMAVAGMPTAYDLSSVIA SGSSVGHNNLIPLANTGIVN HTHSRMGSIMSTGIVQGSSG AQGSGGGSTSAHYAVNSQFT MGGPAISMASPMSIPTNT |
| Tissue Specificity | Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. |
| Post Translational Modifications | Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing. Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol. Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity. Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO9 at Lys-227.leading to proteasomal degradation. |
| Function | Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activation of transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as a coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. Acts as a transcriptional coactivator of ACACA/acetyl-CoA carboxylase by enriching H3R17 methylation at its promoter, thereby positively regulating fatty acid synthesis. Independently of its methyltransferase activity, involved in replication fork progression: promotes PARP1 recruitment to replication forks, leading to poly-ADP-ribosylation of chromatin at replication forks and reduced fork speed. |
| Protein Name | Histone-Arginine Methyltransferase Carm1 Coactivator-Associated Arginine Methyltransferase 1 Protein Arginine N-Methyltransferase 4 |
| Database Links | Reactome: R-HSA-1368082 Reactome: R-HSA-1368108 Reactome: R-HSA-1989781 Reactome: R-HSA-2151201 Reactome: R-HSA-2426168 Reactome: R-HSA-3214858 Reactome: R-HSA-381340 Reactome: R-HSA-400206 Reactome: R-HSA-400253 Reactome: R-HSA-6804114 Reactome: R-HSA-9018519 Reactome: R-HSA-9707564 Reactome: R-HSA-9707616 |
| Cellular Localisation | Nucleus Cytoplasm Chromosome Mainly Nuclear During The G1 S And G2 Phases Of The Cell Cycle Cytoplasmic During Mitosis After Breakup Of The Nuclear Membrane Localizes To Replication Forks |
| Alternative Antibody Names | Anti-Histone-Arginine Methyltransferase Carm1 antibody Anti-Coactivator-Associated Arginine Methyltransferase 1 antibody Anti-Protein Arginine N-Methyltransferase 4 antibody Anti-CARM1 antibody Anti-PRMT4 antibody |
Information sourced from Uniprot.org