• Western blot analysis of CTNI. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.
  • Immunohistochemistry analysis of CTNI. Sample: mouse heart, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of CTNI. Sample: Rat heart, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of CTNI. Sample: mouse myocardial infarction model heart, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of CTNI. Sample: Rat myocardial infarction model heart, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-Cardiac Troponin I antibody [SM5080] (STJA0005080)

SKU:
STJA0005080

£170.50 - £233.50
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Host: Mouse
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Cardiac Troponin I is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM5080
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (M, R) 1:500-1:1000
IHC/IF (M, R) 1:500-1:1000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: TNNI3
Gene ID: 7137
Uniprot ID: TNNI3_HUMAN
Immunogen: KLH conjugated Synthetic peptide corresponding to Human CTNI
Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Name Troponin I - Cardiac Muscle
Cardiac Troponin I
Database Links Reactome: R-HSA-390522
Reactome: R-HSA-5578775
Alternative Antibody Names Anti-Troponin I - Cardiac Muscle antibody
Anti-Cardiac Troponin I antibody
Anti-TNNI3 antibody
Anti-TNNC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance