| Host: | Rabbit |
| Applications: | WB/IHC-P/ICC/IF/IP |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-Cardiac Troponin I for use in WB, IHC-P, ICC, IF and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R04-1D7 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification: | Affinity Chromatography |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:50 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | TNNI3 |
| Gene ID: | 7137 |
| Uniprot ID: | TNNI3_HUMAN |
| Immunogen: | A synthesized peptide derived from human Troponin I |
| Post Translational Modifications | Phosphorylated at Ser-42 and Ser-44 by PRKCE.phosphorylation increases myocardium contractile dysfunction. Phosphorylated at Ser-23 and Ser-24 by PRKD1.phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Protein Name | Troponin I - Cardiac MuscleCardiac Troponin I |
| Database Links | Reactome: R-HSA-390522Reactome: R-HSA-5578775 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Troponin I - Cardiac Muscle antibodyAnti-Cardiac Troponin I antibodyAnti-TNNI3 antibodyAnti-TNNC1 antibody |
Information sourced from Uniprot.org
