| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CAPN3 (70-309) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CAPN3 |
| Gene ID: |
825 |
| Uniprot ID: |
CAN3_HUMAN |
| Immunogen Region: |
70-309 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 70-309 of human CAPN3 (NP_775111.1). |
| Immunogen Sequence: |
EEVENTISVDRPVKKKKTKP IIFVSDRANSNKELGVDQES EEGKGKTSPDKQKQSPQPQP GSSDQESEEQQQFRNIFKQI AGDDMEICADELKKVLNTVV NKHKDLKTHGFTLESCRSMI ALMDTDGSGKLNLQEFHHLW NKIKAWQKIFKHYDTDQSGT INSYEMRNAVNDAGFHLNNQ LYDIITMRYADKHMNIDFDS FICCFVRLEGMFRAFHAFDK DGDGIIKLNVLEWLQLTMY |
| Tissue Specificity | Isoform I is skeletal muscle specific. |
| Function | Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-409'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53. |
| Protein Name | Calpain-3Calcium-Activated Neutral Proteinase 3Canp 3Calpain L3Calpain P94Muscle-Specific Calcium-Activated Neutral Protease 3New Calpain 1Ncl-1 |
| Database Links | Reactome: R-HSA-1474228 |
| Cellular Localisation | CytoplasmNucleusNucleolus |
| Alternative Antibody Names | Anti-Calpain-3 antibodyAnti-Calcium-Activated Neutral Proteinase 3 antibodyAnti-Canp 3 antibodyAnti-Calpain L3 antibodyAnti-Calpain P94 antibodyAnti-Muscle-Specific Calcium-Activated Neutral Protease 3 antibodyAnti-New Calpain 1 antibodyAnti-Ncl-1 antibodyAnti-CAPN3 antibodyAnti-CANP3 antibodyAnti-CANPL3 antibodyAnti-NCL1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TOMM34 knockout (KO) HeLa cells, using [KO Validated] TOMM34 Rabbit polyclonal antibody (STJ11100836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89771/358243/STJ11100836_1__34745.1713122577.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TOMM34 knockout (KO) HeLa cells, using [KO Validated] TOMM34 Rabbit polyclonal antibody (STJ11100836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")