• Western blot analysis of extracts of various cell lines, using CAPN3 Antibody (STJ113898) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of L929 cells using CAPN3 rabbit polyclonal antibody (STJ113898) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CAPN3 antibody (1-150) (STJ113898)

SKU:
STJ113898

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAPN3 (1-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAPN3
Gene ID: 825
Uniprot ID: CAN3_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CAPN3 (NP_775111.1).
Immunogen Sequence: MHGNKQHLQKDFFLYNASKA RSKTYINMREVSQRFRLPPS EYVIVPSTYEPHQEGEFILR VFSEKRNLSEEVENTISVDR PVKKKKTKPIIFVSDRANSN KELGVDQESEEGKGKTSPDK QKQSPQPQPGSSDQESEEQQ QFRNIFKQIA
Tissue Specificity Isoform I is skeletal muscle specific.
Function Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-409'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53.
Protein Name Calpain-3
Calcium-Activated Neutral Proteinase 3
Canp 3
Calpain L3
Calpain P94
Muscle-Specific Calcium-Activated Neutral Protease 3
New Calpain 1
Ncl-1
Database Links Reactome: R-HSA-1474228
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Alternative Antibody Names Anti-Calpain-3 antibody
Anti-Calcium-Activated Neutral Proteinase 3 antibody
Anti-Canp 3 antibody
Anti-Calpain L3 antibody
Anti-Calpain P94 antibody
Anti-Muscle-Specific Calcium-Activated Neutral Protease 3 antibody
Anti-New Calpain 1 antibody
Anti-Ncl-1 antibody
Anti-CAPN3 antibody
Anti-CANP3 antibody
Anti-CANPL3 antibody
Anti-NCL1 antibody

Information sourced from Uniprot.org

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