Anti-CAPN1 antibody (355-530) (STJ22880)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CAPN1 (355-530) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CAPN1 |
| Gene ID | 823 |
| Uniprot ID | CAN1_HUMAN |
| Immunogen Region | 355-530 |
| Immunogen Sequence | PDALKSRTIRKWNTTLYEGT WRRGSTAGGCRNYPATFWVN PQFKIRLDETDDPDDYGDRE SGCSFVLALMQKHRRRERRF GRDMETIGFAVYEVPPELVG QPAVHLKRDFFLANASRARS EQFINLREVSTRFRLPPGEY VVVPSTFEPNKEGDFVLRFF SEKSAGTVELDDQIQA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 355-530 of human Calpain 1 (NP_005177.2). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms. |
| Function | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7). |
| Protein Name | Calpain-1 Catalytic SubunitCalcium-Activated Neutral Proteinase 1Canp 1Calpain Mu-TypeCalpain-1 Large SubunitCell Proliferation-Inducing Gene 30 ProteinMicromolar-CalpainMucanp |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-6798695Reactome: R-HSA-6809371Reactome: R-HSA-8862803 |
| Cellular Localisation | CytoplasmCell MembraneTranslocates To The Plasma Membrane Upon Ca(2+) BindingIn Granular Keratinocytes And In Lower CorneocytesColocalizes With Flg And Flg2 |
| Alternative Antibody Names | Anti-Calpain-1 Catalytic Subunit antibodyAnti-Calcium-Activated Neutral Proteinase 1 antibodyAnti-Canp 1 antibodyAnti-Calpain Mu-Type antibodyAnti-Calpain-1 Large Subunit antibodyAnti-Cell Proliferation-Inducing Gene 30 Protein antibodyAnti-Micromolar-Calpain antibodyAnti-Mucanp antibodyAnti-CAPN1 antibodyAnti-CANPL1 antibodyAnti-PIG30 antibody |
Information sourced from Uniprot.org