| Short Description : | Goat polyclonal antibody anti-CAPG (Internal) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications. |
| Applications: | Pep-ELISA/WB/FC |
| Host: | Goat |
| Reactivity: | Human/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 38kDa band observed in lysates of cell lines THP-1, U937 and A431, and in Human Spleen lysates and approx. 37kDa in Human Tonsil lysates (calculated MW of 38.5kDa according to |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CAPG |
| Gene ID: | 822 |
| Uniprot ID: | CAPG_HUMAN |
| Accession Number: | NP_001738.2; NP_001243069.1 |
| Immunogen Region: | Internal |
| Immunogen Sequence: | NIRATERALNWDS |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_001738.2; NP_001243069.1). Reported variants represent identical protein: NP_001243068.1, NP_001738.2. |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. |
| Protein Name | Macrophage-Capping ProteinActin Regulatory Protein Cap-G |
| Cellular Localisation | NucleusCytoplasmMelanosomeCell ProjectionLamellipodiumRuffleIn MacrophagesMay Be Predominantly CytoplasmicNuclear Localization Was Observed In FibroblastsPresent At The Membrane-Cytoplasm InterfaceIn Activated MacrophagesConcentrated In The Ruffles Of The Leading Lamellipodia |
| Alternative Antibody Names | Anti-Macrophage-Capping Protein antibodyAnti-Actin Regulatory Protein Cap-G antibodyAnti-CAPG antibodyAnti-AFCP antibodyAnti-MCP antibody |
Information sourced from Uniprot.org
