| Host: |
Rabbit |
| Applications: |
IHC/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Calmodulin-Regulated Spectrin-Associated Protein 3 is suitable for use in Immunohistochemistry and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4 |
| Purification: |
Antigen affinity purification |
| Concentration: |
1.32 mg/mL |
| Dilution Range: |
IHC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Function | Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization. Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization. Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization. In addition, it also reduces the velocity of microtubule polymerization. Required for the biogenesis and the maintenance of zonula adherens by anchoring the minus-end of microtubules to zonula adherens and by recruiting the kinesin KIFC3 to those junctional sites. Required for orienting the apical-to-basal polarity of microtubules in epithelial cells: acts by tethering non-centrosomal microtubules to the apical cortex, leading to their longitudinal orientation. Plays a key role in early embryos, which lack centrosomes: accumulates at the microtubule bridges that connect pairs of cells and enables the formation of a non-centrosomal microtubule-organizing center that directs intracellular transport in the early embryo. Couples non-centrosomal microtubules with actin: interaction with MACF1 at the minus ends of non-centrosomal microtubules, tethers the microtubules to actin filaments, regulating focal adhesion size and cell migration. Plays a key role in the generation of non-centrosomal microtubules by accumulating in the pericentrosomal region and cooperating with KATNA1 to release non-centrosomal microtubules from the centrosome. Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes. Through interaction with AKAP9, involved in translocation of Golgi vesicles in epithelial cells, where microtubules are mainly non-centrosomal. Plays an important role in motile cilia function by facilitatating proper orientation of basal bodies and formation of central microtubule pairs in motile cilia. |
| Protein Name | Calmodulin-Regulated Spectrin-Associated Protein 3Protein Nezha |
| Cellular Localisation | CytoplasmCytoskeletonCell JunctionAdherens JunctionCilium AxonemeCilium Basal BodyScattered In The CytoplasmAssociated With The Minus-End Of Microtubules And Also Detected At The CentrosomesDecorates The Minus-End Of Microtubules By Decreasing The Rate Of Tubulin Incorporation And Remaining BoundLocalizes Along Zonula Adherens Only At Mature Cell-Cell ContactsIn Early EmbryosAccumulates At The Microtubule Bridges That Connect Pairs Of Cells: This Structure Is Present In Early EmbryosWhich Lack CentrosomesThis Cytokinetic Bridge Does Not Undergo Stereotypical Abscission After Cell DivisionAccumulates To The Pericentrosomal Region Following Interaction With Katna1 |
| Alternative Antibody Names | Anti-Calmodulin-Regulated Spectrin-Associated Protein 3 antibodyAnti-Protein Nezha antibodyAnti-CAMSAP3 antibodyAnti-KIAA1543 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
