• Immunofluorescence analysis of NIH-3T3 cells using CALU antibody (STJ28621) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using CALU antibody (STJ28621) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using CALU antibody (STJ28621) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse skin using CALU antibody (STJ28621) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CALU antibody (STJ28621) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Basic Kit. _Exposure time: 90s.

Anti-CALU antibody (20-315) (STJ28621)

SKU:
STJ28621

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CALU (20-315) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALU
Gene ID: 813
Uniprot ID: CALU_HUMAN
Immunogen Region: 20-315
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-315 of human CALU (NP_001210.1).
Immunogen Sequence: KPTEKKDRVHHEPQLSDKVH NDAQSFDYDHDAFLGAEEAK TFDQLTPEESKERLGKIVSK IDGDKDGFVTVDELKDWIKF AQKRWIYEDVERQWKGHDLN EDGLVSWEEYKNATYGYVLD DPDPDDGFNYKQMMVRDERR FKMADKDGDLIATKEEFTAF LHPEEYDYMKDIVVQETMED IDKNADGFIDLEEYIGDMYS HDGNTDEPEWVKTEREQFVE FRDKNRDGKMDKEETKDWI
Tissue Specificity Ubiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver.
Function Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.
Protein Name Calumenin
Crocalbin
Ief Ssp 9302
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Endoplasmic Reticulum Membrane
Golgi Apparatus
Secreted
Melanosome
Sarcoplasmic Reticulum Lumen
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Calumenin antibody
Anti-Crocalbin antibody
Anti-Ief Ssp 9302 antibody
Anti-CALU antibody

Information sourced from Uniprot.org

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