• Western blot analysis of lysates from mouse liver using CA5A rabbit polyclonal antibody (STJ116909) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:5s.
  • Immunofluorescence analysis of NIH/3T3 cells using CA5A rabbit polyclonal antibody (STJ116909) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using CA5A rabbit polyclonal antibody (STJ116909) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CA5A antibody (39-305) (STJ116909)

SKU:
STJ116909

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CA5A (39-305) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CA5A
Gene ID: 763
Uniprot ID: CAH5A_HUMAN
Immunogen Region: 39-305
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 39-305 of human CA5A (NP_001730.1).
Immunogen Sequence: CAWQTSNNTLHPLWTVPVSV PGGTRQSPINIQWRDSVYDP QLKPLRVSYEAASCLYIWNT GYLFQVEFDDATEASGISGG PLENHYRLKQFHFHWGAVNE GGSEHTVDGHAYPAELHLVH WNSVKYQNYKEAVVGENGLA VIGVFLKLGAHHQTLQRLVD ILPEIKHKDARAAMRPFDPS TLLPTCWDYWTYAGSLTTPP LTESVTWIIQKEPVEVAPSQ LSAFRTLLFSALGEEEKMM
Function Mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3. Mitochondria are impermeable to HCO3, and thus this intramitochondrial carbonic anhydrase is pivotal in providing HCO3 for multiple mitochondrial enzymes that catalyze the formation of essential metabolites of intermediary metabolism in the urea and Krebs cycles.
Protein Name Carbonic Anhydrase 5a - Mitochondrial
Carbonate Dehydratase Va
Carbonic Anhydrase Va
Ca-Va
Database Links Reactome: R-HSA-1475029
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Carbonic Anhydrase 5a - Mitochondrial antibody
Anti-Carbonate Dehydratase Va antibody
Anti-Carbonic Anhydrase Va antibody
Anti-Ca-Va antibody
Anti-CA5A antibody
Anti-CA5 antibody

Information sourced from Uniprot.org

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