Anti-C9 antibody (205-216) (STJ73367)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-C9 (205-216) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 70kDa band observed in Human Placenta, Kidney and Liver lysates (calculated MW of 63.2kDa according to NP_001728.1). The observed molecular weight corresponds to the glycosylated |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | C9 |
| Gene ID | 735 |
| Uniprot ID | CO9_HUMAN |
| Accession Number | NP_001728.1 |
| Immunogen Region | 205-216 |
| Immunogen Sequence | ETKGEKNFRTEH |
Additional Info
| Post Translational Modifications | Thrombin cleaves factor C9 to produce C9a and C9b. Phosphorylation sites are present in the extracellular medium. Initially, positions and connectivity of disulfide bonds were based on peptide sequencing done for the human protein. The crystal structures for the human and mouse proteins corrected the positions and connectivities of the disulfide bonds. The distance between Cys-57 and Cys-94 in the monomeric mouse protein precludes formation of a disulfide bond, contrary to what is seen in the structure of the human polymeric form of the protein (Probable). |
| Function | Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC. |
| Protein Name | Complement Component C9 Cleaved Into - Complement Component C9a - Complement Component C9b |
| Database Links | Reactome: R-HSA-166665Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedTarget Cell MembraneMulti-Pass Membrane ProteinSecreted As Soluble MonomerOligomerizes At Target MembranesForming A Pre-PoreA Conformation Change Then Leads To The Formation Of A 100 Angstrom Diameter Pore |
| Alternative Antibody Names | Anti-Complement Component C9 Cleaved Into - Complement Component C9a - Complement Component C9b antibodyAnti-C9 antibody |
Information sourced from Uniprot.org