| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | C3 |
| Gene ID: | 12266 |
| Uniprot ID: | CO3_MOUSE |
| Immunogen Region: | 1159-1286 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1159-1286 of mouse C3 (NP_033908.2). |
| Immunogen Sequence: | EGQVNSLPGSINKAGEYIEA SYMNLQRPYTVAIAGYALAL MNKLEEPYLGKFLNTAKDRN RWEEPDQQLYNVEATSYALL ALLLLKDFDSVPPVVRWLNE QRYYGGGYGSTQATFMVFQA LAQYQTDV |
| Post Translational Modifications | C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by dietary chylomicrons. Phosphorylated by FAM20C in the extracellular medium. |
| Function | C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. The short isoform has B-cell stimulatory activity. C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein: Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2. |
| Protein Name | Complement C3 Hse-Msf Cleaved Into - Complement C3 Beta Chain - C3-Beta-C C3bc - Complement C3 Alpha Chain - C3a Anaphylatoxin - Acylation Stimulating Protein Asp C3adesarg - Complement C3b Alpha' Chain - Complement C3c Alpha' Chain Fragment 1 - Complement C3dg Fragment - Complement C3g Fragment - Complement C3d Fragment - Complement C3f Fragment - Complement C3c Alpha' Chain Fragment 2 |
| Database Links | Reactome: R-MMU-173736 Reactome: -MMU-174577 Reactome: -MMU-198933 Reactome: -MMU-375276 Reactome: -MMU-381426 Reactome: -MMU-418594 Reactome: -MMU-6798695 Reactome: -MMU-8957275 Reactome: -MMU-977606 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Complement C3 antibody Anti-Hse-Msf Cleaved Into - Complement C3 Beta Chain - C3-Beta-C antibody Anti-C3bc - Complement C3 Alpha Chain - C3a Anaphylatoxin - Acylation Stimulating Protein antibody Anti-Asp antibody Anti-C3adesarg - Complement C3b Alpha' Chain - Complement C3c Alpha' Chain Fragment 1 - Complement C3dg Fragment - Complement C3g Fragment - Complement C3d Fragment - Complement C3f Fragment - Complement C3c Alpha' Chain Fragment 2 antibody Anti-C3 antibody |
Information sourced from Uniprot.org