| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
Rlig1 |
| Gene ID: |
68281 |
| Uniprot ID: |
RLIG1_MOUSE |
| Immunogen Region: |
1-220 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of mouse C12orf29 (NP_780337.2). |
| Immunogen Sequence: |
MKRLGSVQRKMPCVFVTEVK AEPSAKREHQPFKVLATETL SEKALDADVYNAVATEKVDG TCCYVTNYKGQPYLWARLDR KPNKQADKRFKKFLHSKESA KEFHWNTEEDFKPVPECWIP AKEIEKQNGKPVPDENGHIP GWVPVEKGSKQYCWHSSVVN YEFGIALVLRHHPDDPGVLE ISAVPLSELLEQTLELIGTS INGNPYGLGSKKSPLHFLTP |
| Post Translational Modifications | AMPylates itself (auto-AMPylation). |
| Function | Functions as an RNA ligase, in vitro. The ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate. In step 2, the ligase-AMP binds to the nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated intermediate. In step 3, the RNA ligase directs the attack of the 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3'-5' phosphodiester and release of AMP. Exhibits selectivity for single-stranded RNA substrates and may not have nick-sealing activity on double-stranded DNA-RNA hybrids. May play a role in maintaining RNA integrity under stress conditions, for example in response to reactive oxygen species (ROS). |
| Protein Name | Rna Ligase 1Rna LigaseRnl |
| Alternative Antibody Names | Anti-Rna Ligase 1 antibodyAnti-Rna Ligase antibodyAnti-Rnl antibodyAnti-Rlig1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
