• Immunofluorescence analysis of C6 cells using Beta-TrCP/BTRC Rabbit polyclonal antibody (STJ22845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using Beta-TrCP/BTRC Rabbit polyclonal antibody (STJ22845) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of U-251MG cells, using Beta-TrCP/BTRC antibody (STJ22845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-BTRC antibody (1-120) (STJ22845)

SKU:
STJ22845

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Beta-TRCP (1-120) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BTRC
Gene ID: 8945
Uniprot ID: FBW1A_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Beta-TrCP/BTRC (NP_378663.1).
Immunogen Sequence: MDPAEAVLQEKALKFMCSMP RSLWLGCSSLADSMPSLRCL YNPGTGALTAFQNSSEREDC NNGEPPRKIIPEKNSLRQTY NSCARLCLNQETVCLASTAM KTENCVAKTKLANGTSSMIV
Tissue Specificity Expressed in epididymis (at protein level).
Post Translational Modifications Ubiquitinated. Deubiquitinated by OTUD5, promoting its stability.
Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE.the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. The SCF(FBXW11) complex also regulates NF-kappa-B by mediating ubiquitination of phosphorylated NFKB1: specifically ubiquitinates the p105 form of NFKB1, leading to its degradation. SCF(BTRC) mediates the ubiquitination of CEP68.this is required for centriole separation during mitosis. SCF(BTRC) mediates the ubiquitination and subsequent degradation of nuclear NFE2L1. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3. Mediates ubiquitination of REST, thereby leading to its proteasomal degradation. SCF(BTRC) mediates the ubiquitination and subsequent proteasomal degradation of KLF4.thereby negatively regulating cell pluripotency maintenance and embryogenesis. SCF(BTRC) acts as a regulator of mTORC1 signaling pathway by catalyzing ubiquitination and subsequent proteasomal degradation of phosphorylated DEPTOR, TFE3 and MITF.
Protein Name F-Box/Wd Repeat-Containing Protein 1a
E3rsikappab
Epididymis Tissue Protein Li 2a
F-Box And Wd Repeats Protein Beta-Trcp
Pikappabalpha-E3 Receptor Subunit
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1170546
Reactome: R-HSA-174113
Reactome: R-HSA-180534
Reactome: R-HSA-195253
Reactome: R-HSA-202424
Reactome: R-HSA-2565942
Reactome: R-HSA-2871837
Reactome: R-HSA-3769402
Reactome: R-HSA-400253
Reactome: R-HSA-5607761
Reactome: R-HSA-5607764
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5676590
Reactome: R-HSA-5684264
Reactome: R-HSA-8951664
Reactome: R-HSA-9020702
Reactome: R-HSA-9762114
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-F-Box/Wd Repeat-Containing Protein 1a antibody
Anti-E3rsikappab antibody
Anti-Epididymis Tissue Protein Li 2a antibody
Anti-F-Box And Wd Repeats Protein Beta-Trcp antibody
Anti-Pikappabalpha-E3 Receptor Subunit antibody
Anti-BTRC antibody
Anti-BTRCP antibody
Anti-FBW1A antibody
Anti-FBXW1A antibody

Information sourced from Uniprot.org

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