| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BSN |
| Gene ID: | 8927 |
| Uniprot ID: | BSN_HUMAN |
| Immunogen Region: | 830-950 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 830-950 of human BSN (NP_003449.2). |
| Immunogen Sequence: | PPARAAELTDEDFMRRQILE MSAEEDNLEEDDTATSGRGL AKHGTQKGGPRPRPEPSQEP AALPKRRLPHNATTGYEELL PEGGSAEATDGSGTLQGGLR RFKTIELNSTGSYGHELDLG Q |
| Tissue Specificity | Exclusively expressed in brain. |
| Post Translational Modifications | Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization. |
| Function | Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released. After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts. At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis. Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5. Mediates also synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import. Inhibits the activity of the proportion of DAO enzyme that localizes to the presynaptic active zone, which may modulate synaptic transmission. |
| Protein Name | Protein Bassoon Zinc Finger Protein 231 |
| Database Links | Reactome: R-HSA-9662360 |
| Cellular Localisation | Cytoplasm Presynaptic Active Zone Cytoskeleton Cytoplasmic Vesicle Secretory Vesicle Synaptic Vesicle Membrane Peripheral Membrane Protein In Retina Is Localized In The Outer Plexiform Layer At Ribbon Synapses Formed By Rods And Cones But Was Absent From Basal Synaptic Contacts Formed By Cones In The Retinal Inner Plexiform Layer Localized To Conventional Inhibitory Gabaergic Synapses Made By Amacrine Cells But Absent From The Bipolar Cell Ribbon Synapses |
| Alternative Antibody Names | Anti-Protein Bassoon antibody Anti-Zinc Finger Protein 231 antibody Anti-BSN antibody Anti-KIAA0434 antibody Anti-ZNF231 antibody |
Information sourced from Uniprot.org