• Western blot analysis of lysates from 293 cells, using BRSK1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from COLO205 cells using BRSK1 antibody.
  • Western blot analysis of various cells using BRSK1 Polyclonal Antibody

Anti-BRSK1 antibody (361-410 aa) (STJ91900)

SKU:
STJ91900

Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Serine/threonine-protein kinase BRSK1 (361-410 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRSK1
Gene ID: 84446
Uniprot ID: BRSK1_HUMAN
Immunogen Region: 361-410 aa
Specificity: BRSK1 Polyclonal Antibody detects endogenous levels of BRSK1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BRSK1 at the amino acid range 361-410
Post Translational Modifications Phosphorylated at Thr-189 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not phosphorylated at Thr-189 by CaMKK2. In contrast, it is phosphorylated and activated by CaMKK1. May be inactivated via dephosphorylation of Thr-189 by PP2C.
Function Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.
Protein Name Serine/Threonine-Protein Kinase Brsk1
Brain-Selective Kinase 1
Brain-Specific Serine/Threonine-Protein Kinase 1
Br Serine/Threonine-Protein Kinase 1
Serine/Threonine-Protein Kinase Sad-B
Synapses Of Amphids Defective Homolog 1
Sad1 Homolog
Hsad1
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Microtubule Organizing Center
Centrosome
Synapse
Presynaptic Active Zone
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle
Nuclear In The Absence Of Dna Damage
Translocated To The Nucleus In Response To Uv- Or Mms-Induced Dna Damage
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Brsk1 antibody
Anti-Brain-Selective Kinase 1 antibody
Anti-Brain-Specific Serine/Threonine-Protein Kinase 1 antibody
Anti-Br Serine/Threonine-Protein Kinase 1 antibody
Anti-Serine/Threonine-Protein Kinase Sad-B antibody
Anti-Synapses Of Amphids Defective Homolog 1 antibody
Anti-Sad1 Homolog antibody
Anti-Hsad1 antibody
Anti-BRSK1 antibody
Anti-KIAA1811 antibody
Anti-SAD1 antibody
Anti-SADB antibody

Information sourced from Uniprot.org

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