Anti-BNIP3 antibody [R09-1G8] (STJA0033937)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human BNIP3
STJA0033937
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General Information

Short DescriptionRabbit monoclonal anti-BNIP3 for use in WB, ICC and IF in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ICC/IF
HostRabbit
ReactivityHuman/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR09-1G8
IsotypeIgG
ConjugationUnconjugated
Concentration0.3 mg/mL
PurificationAffinity Purified
Dilution RangeWB 1:500-1:1000
IF 1:50-1:200
Formulation50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolBNIP3
Gene ID664
Uniprot IDBNIP3_HUMAN
ImmunogenA synthetic peptide of human BNIP3

Additional Info

Function Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
Protein Name Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 3
Database Links
Cellular Localisation Mitochondrion
Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Coexpression With The Eib 19-Kda Protein Results In A Shift In Nip3 Localization Pattern To The Nuclear Envelope
Colocalizes With Acaa2 In The Mitochondria
Colocalizes With Spata18 At The Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 3 antibody
Anti-BNIP3 antibody
Anti-NIP3 antibody

Information sourced from Uniprot.org

Citations

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