• Immunoprecipitation analysis of 200 Mu g extracts of K-562 cells, using 3 Mu g Bmi1 antibody (STJ22809). Western blot was performed from the immunoprecipitate using Bmi1 antibody (STJ22809) at a dilution of 1:1000.
  • Western blot analysis of various lysates using Bmi1 Rabbit polyclonal antibody (STJ22809) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using Bmi1 Rabbit polyclonal antibody (STJ22809) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BMI1 antibody (200-300) (STJ22809)

SKU:
STJ22809

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BMI1 (200-300) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BMI1
Gene ID: 100532731/648
Uniprot ID: BMI1_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Bmi1 (NP_005171.4).
Immunogen Sequence: LKDYYTLMDIAYIYTWRRNG PLPLKYRVRPTCKRMKISHQ RDGLTNAGELESDSGSDKAN SPAGGIPSTSSCLPSPSTPV QSPHPQFPHISSTMNGTSNS P
Post Translational Modifications Monoubiquitinated. May be polyubiquitinated.which does not lead to proteasomal degradation.
Function Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Protein Name Polycomb Complex Protein Bmi-1
Polycomb Group Ring Finger Protein 4
Ring Finger Protein 51
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-3108214
Reactome: R-HSA-3899300
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4655427
Reactome: R-HSA-8939243
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Polycomb Complex Protein Bmi-1 antibody
Anti-Polycomb Group Ring Finger Protein 4 antibody
Anti-Ring Finger Protein 51 antibody
Anti-BMI1 antibody
Anti-PCGF4 antibody
Anti-RNF51 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance