• Western blot analysis of lysates from Jurkat cells, using BLZF1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from COLO205 cells using BLZF1 antibody.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-BLZF1 antibody (10-59 aa) (STJ93307)

SKU:
STJ93307

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Golgin-45 (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BLZF1
Gene ID: 8548
Uniprot ID: GO45_HUMAN
Immunogen Region: 10-59 aa
Specificity: Golgin 45 Polyclonal Antibody detects endogenous levels of Golgin 45 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BLZF1 at the amino acid range 10-59
Post Translational Modifications ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.
Function Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
Protein Name Golgin-45
Basic Leucine Zipper Nuclear Factor 1
Jem-1
P45 Basic Leucine-Zipper Nuclear Factor
Database Links Reactome: R-HSA-162658
Cellular Localisation Golgi Apparatus Membrane
Isoform 1: Nucleus
Detected In The Nucleus Upon Heterologous Expression
Not Detected In The Cytoplasm
Isoform 2: Cytoplasm
Not Detected In The Nucleus
Alternative Antibody Names Anti-Golgin-45 antibody
Anti-Basic Leucine Zipper Nuclear Factor 1 antibody
Anti-Jem-1 antibody
Anti-P45 Basic Leucine-Zipper Nuclear Factor antibody
Anti-BLZF1 antibody
Anti-JEM1 antibody

Information sourced from Uniprot.org

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