Anti-BLNK/SLP-65 antibody (C-Term) (STJ70040)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-BLNK/SLP-65 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/WB/IF/FC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70-75kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to NP_037446.1). This molecular weight is routinely observed by other sources. Recommend |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | BLNK |
| Gene ID | 29760 |
| Uniprot ID | BLNK_HUMAN |
| Accession Number | NP_037446.1; NP_001107566.1; NP_001245369.1; NP_001245370.1; NP_001245371.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | KDSTRLKYAVKVS |
| Specificity | This antibody is expected to recognize all reported isoforms. |
Additional Info
| Post Translational Modifications | Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways. |
| Function | Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis. |
| Protein Name | B-Cell Linker ProteinB-Cell Adapter Containing A Sh2 Domain ProteinB-Cell Adapter Containing A Src Homology 2 Domain ProteinCytoplasmic Adapter ProteinSrc Homology 2 Domain-Containing Leukocyte Protein Of 65 KdaSlp-65 |
| Database Links | Reactome: R-HSA-912631Reactome: R-HSA-9679191Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmCell MembraneBcr Activation Results In The Translocation To Membrane Fraction |
| Alternative Antibody Names | Anti-B-Cell Linker Protein antibodyAnti-B-Cell Adapter Containing A Sh2 Domain Protein antibodyAnti-B-Cell Adapter Containing A Src Homology 2 Domain Protein antibodyAnti-Cytoplasmic Adapter Protein antibodyAnti-Src Homology 2 Domain-Containing Leukocyte Protein Of 65 Kda antibodyAnti-Slp-65 antibodyAnti-BLNK antibodyAnti-BASH antibodyAnti-SLP65 antibody |
Information sourced from Uniprot.org