Anti-BLM antibody (65-114 aa) (STJ91858)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Bloom Syndrome at the amino acid range 65-114
General Information
| Short Description | Rabbit polyclonal anti-RecQ-like DNA helicase BLM (65-114 aa) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BLM |
| Gene ID | 641 |
| Uniprot ID | BLM_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Bloom Syndrome at the amino acid range 65-114 |
| Immunogen Region | 65-114 aa |
| Specificity | BLM Polyclonal Antibody detects endogenous levels of BLM protein. |
Additional Info
| Function | ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction. Participates in DNA replication and repair. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and Holliday junction DNA. Unwinds G-quadruplex DNA.unwinding occurs in the 3'-5' direction and requires a 3' single-stranded end of at least 7 nucleotides. Helicase activity is higher on G-quadruplex substrates than on duplex DNA substrates. Telomeres, immunoglobulin heavy chain switch regions and rDNA are notably G-rich.formation of G-quadruplex DNA would block DNA replication and transcription. Negatively regulates sister chromatid exchange (SCE). Recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks. (Microbial infection) Eliminates nuclear HIV-1 cDNA, thereby suppressing immune sensing and proviral hyper-integration. |
| Protein Name | Recq-Like Dna Helicase BlmBloom Syndrome ProteinDna 3'-5' Helicase BlmDna Helicase - Recq-Like Type 2Recq2Recq Protein-Like 3 |
| Database Links | Reactome: R-HSA-174414Reactome: R-HSA-3108214Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-912446Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusTogether With SpidrIs Redistributed In Discrete Nuclear Dna Damage-Induced Foci Following Hydroxyurea (Hu) Or Camptothecin (Cpt) TreatmentAccumulated At Sites Of Dna Damage In A Rmi Complex- And Spidr-Dependent Manner |
| Alternative Antibody Names | Anti-Recq-Like Dna Helicase Blm antibodyAnti-Bloom Syndrome Protein antibodyAnti-Dna 3'-5' Helicase Blm antibodyAnti-Dna Helicase - Recq-Like Type 2 antibodyAnti-Recq2 antibodyAnti-Recq Protein-Like 3 antibodyAnti-BLM antibodyAnti-RECQ2 antibodyAnti-RECQL3 antibody |
Information sourced from Uniprot.org