• Western blot analysis of lysates from Rosette leaves, using BIN2 Rabbit polyclonal antibody (STJ11104219) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BIN2 antibody (281-380) (STJ11104219)

SKU:
STJ11104219

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Host: Rabbit
Applications: WB/ELISA
Reactivity: A.thaliana
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 281-380
Specificity: A synthetic peptide corresponding to a sequence within amino acids 281-380 of arabidopsis thaliana BIN2 (NP_193606.1).
Immunogen Sequence: KAHPWHKIFHKRMPPEAIDF ASRLLQYSPSLRCTALEACA HPFFDELREPNARLPNGRPF PPLFNFKQEVAGSSPELVNK LIPDHIKRQLGLSFLNQSGT
Background Encodes BIN2, a member of the ATSK (shaggy-like kinase) family. BIN2 functions in the cross-talk between auxin and brassinosteroid signaling pathways. BIN2 regulates root epidermal cell fate specification by phosphorylating EGL3 and TTG1. BIN2-mediated phosphorylation appears to promote BZR1 export from the nucleus.

Information sourced from Uniprot.org

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