| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-BIM (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 23kDa band observed in lysates of cell line K562 (calculated MW of 18.5kDa according to NP_619528.1). Recommended for use at 0.5-2µg/ml. Primary incubated 1 hour at room tempera |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | BCL2L11 |
| Gene ID: | 10018 |
| Uniprot ID: | B2L11_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_619528.1; NP_619529.1; NP_996886.1;Isotype=IgG" |
| Specificity: | This antibody is expected to recognise at least reported isoforms Bim-alpha1 (BimABCD) , Bim-alpha2 (BimACD) and Bim-alpha3 (BimAD). |
| Immunogen Sequence: | FNAYYARRLEK |
| Post Translational Modifications | Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation. Deubiquitination catalyzed by USP27X stabilizes the protein. Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein. |
| Function | Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. |
| Protein Name | Bcl-2-Like Protein 11Bcl2-L-11Bcl2-Interacting Mediator Of Cell Death |
| Database Links | Reactome: R-HSA-111446Reactome: R-HSA-111453Reactome: R-HSA-193648Reactome: R-HSA-6802952Reactome: R-HSA-8862803Reactome: R-HSA-8952158Reactome: R-HSA-9607240Reactome: R-HSA-9614657 |
| Cellular Localisation | Endomembrane SystemPeripheral Membrane ProteinAssociated With Intracytoplasmic MembranesIsoform Bimel: MitochondrionTranslocates From Microtubules To Mitochondria On Loss Of Cell AdherenceIsoform Biml: MitochondrionIsoform Bims: MitochondrionIsoform Bim-Alpha1: Mitochondrion |
| Alternative Antibody Names | Anti-Bcl-2-Like Protein 11 antibodyAnti-Bcl2-L-11 antibodyAnti-Bcl2-Interacting Mediator Of Cell Death antibodyAnti-BCL2L11 antibodyAnti-BIM antibody |
Information sourced from Uniprot.org
