| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-BID (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000. WB: Approx 26+24kDa bands observed in A431 cell lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration: 0.1-0.3µg/ml. Pr |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | BID |
| Gene ID: | 637 |
| Uniprot ID: | BID_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_932070.1; NP_001187.1; NP_932071.1 |
| Specificity: | This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1; NP_001187.1; NP_932071.1) |
| Immunogen Sequence: | TYVRSLARNGMD |
| Post Translational Modifications | BH3-interacting domain death agonist: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products. Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis. BH3-interacting domain death agonist p15: Ubiquitinated by ITCH.ubiquitination results in proteasome-dependent degradation. |
| Function | Induces caspases and apoptosis. Counters the protective effect of BCL2. BH3-interacting domain death agonist p15: Induces caspase activation and apoptosis. Allows the release of cytochrome c. Isoform 1: Induces ICE-like proteases and apoptosis. Isoform 2: Induces ICE-like proteases and apoptosis. Isoform 3: Does not induce apoptosis. Isoform 4: Induces ICE-like proteases and apoptosis. |
| Protein Name | Bh3-Interacting Domain Death Agonist P22 Bid Bid Cleaved Into - Bh3-Interacting Domain Death Agonist P15 P15 Bid - Bh3-Interacting Domain Death Agonist P13 P13 Bid - Bh3-Interacting Domain Death Agonist P11 P11 Bid |
| Database Links | Reactome: R-HSA-111447 Reactome: R-HSA-111452 Reactome: R-HSA-111453 Reactome: R-HSA-114294 Reactome: R-HSA-6803204 Reactome: R-HSA-75108 |
| Cellular Localisation | Cytoplasm Mitochondrion Membrane Mitochondrion Outer Membrane When Uncleaved It Is Predominantly Cytoplasmic Bh3-Interacting Domain Death Agonist P15: Mitochondrion Membrane Translocates To Mitochondria As An Integral Membrane Protein Bh3-Interacting Domain Death Agonist P13: Mitochondrion Membrane Associated With The Mitochondrial Membrane Isoform 1: Cytoplasm Isoform 3: Cytoplasm Isoform 2: Mitochondrion Membrane A Significant Proportion Of Isoform 2 Localizes To Mitochondria It May Be Cleaved Constitutively |
| Alternative Antibody Names | Anti-Bh3-Interacting Domain Death Agonist antibody Anti-P22 Bid antibody Anti-Bid Cleaved Into - Bh3-Interacting Domain Death Agonist P15 antibody Anti-P15 Bid - Bh3-Interacting Domain Death Agonist P13 antibody Anti-P13 Bid - Bh3-Interacting Domain Death Agonist P11 antibody Anti-P11 Bid antibody Anti-BID antibody |
Information sourced from Uniprot.org