• Western blot analysis of extracts of various cell lines, using BHMT Rabbit monoclonal antibody (STJ11102269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180S.

Anti-BHMT antibody (300-406) [S9MR] (STJ11102269)

SKU:
STJ11102269

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-BHMT (300-406) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BHMT
Gene ID: 635
Uniprot ID: BHMT1_HUMAN
Immunogen Region: 300-406
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-406 of human BHMT (Q93088).
Immunogen Sequence: CGFEPYHIRAIAEELAPERG FLPPASEKHGSWGSGLDMHT KPWVRARARKEYWENLRIAS GRPYNPSMSKPDGWGVTKGT AELMQQKEATTEQQLKELFE KQKFKSQ
Tissue Specificity Found exclusively in liver and kidney.
Function Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Protein Name Betaine--Homocysteine S-Methyltransferase 1
Database Links Reactome: R-HSA-1614635
Reactome: R-HSA-6798163
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Predominantly Localized In The Cytoplasm With A Small Fraction Detected In The Nucleus
Translocates Into The Nucleus Upon Oxidative Stress
Alternative Antibody Names Anti-Betaine--Homocysteine S-Methyltransferase 1 antibody
Anti-BHMT antibody

Information sourced from Uniprot.org

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