• Western blot analysis of extracts of Rat liver, using BHMT rabbit polyclonal antibody antibody (STJ22796) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of HepG2 cells, using BHMT rabbit polyclonal antibody antibody (STJ22796) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of human liver cancer cells using BHMT antibody (STJ22796) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-BHMT antibody (177-406) (STJ22796)

SKU:
STJ22796

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BHMT (177-406) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BHMT
Gene ID: 635
Uniprot ID: BHMT1_HUMAN
Immunogen Region: 177-406
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 177-406 of human BHMT (NP_001704.2).
Immunogen Sequence: SGKPVAATMCIGPEGDLHGV PPGECAVRLVKAGASIIGVN CHFDPTISLKTVKLMKEGLE AARLKAHLMSQPLAYHTPDC NKQGFIDLPEFPFGLEPRVA TRWDIQKYAREAYNLGVRYI GGCCGFEPYHIRAIAEELAP ERGFLPPASEKHGSWGSGLD MHTKPWVRARARKEYWENLR IASGRPYNPSMSKPDGWGVT KGTAELMQQKEATTEQQLKE LFEKQKFKSQ
Tissue Specificity Found exclusively in liver and kidney.
Function Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Protein Name Betaine--Homocysteine S-Methyltransferase 1
Database Links Reactome: R-HSA-1614635
Reactome: R-HSA-6798163
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Predominantly Localized In The Cytoplasm With A Small Fraction Detected In The Nucleus
Translocates Into The Nucleus Upon Oxidative Stress
Alternative Antibody Names Anti-Betaine--Homocysteine S-Methyltransferase 1 antibody
Anti-BHMT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance