| Host: |
Rabbit |
| Applications: |
CM/ELISA/IF/IHC/IP/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-BEST1 (500-585) is suitable for use in Confocal Microscopy, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
FITC |
| Isotype: |
IgG |
| Formulation: |
Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: |
Affinity Purified |
| Concentration: |
0.75 µg/µl |
| Dilution Range: |
WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:200IHC: 1:100ICC: 1:100IF: 1:100CFM: 1:100 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
BEST1 |
| Gene ID: |
7439 |
| Uniprot ID: |
BEST1_HUMAN |
| Immunogen Region: |
500-585 |
| Specificity: |
This peptide will label the Bestrophin 1-1-2,-3,-4,-5 isoforms that are produced by alternate splicing of the C-terminal end. |
| Immunogen: |
Synthetic peptide taken within amino acid region 500-585 on human Bestrophin-1 isoform 1 protein. |
| Tissue Specificity | Predominantly expressed in the basolateral membrane of the retinal pigment epithelium. |
| Function | Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+). Allows the movement of chloride and hydrogencarbonate. Found in a partially open conformation leading to significantly smaller chloride movement. Upon F2R/PAR-1 activation, the sequestered calcium is released into the cytosol of astrocytes, leading to the (Ca2+)-dependent release of L-glutamate into the synaptic cleft that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation. Upon activation of the norepinephrine-alpha-1 adrenergic receptor signaling pathway, transports as well D-serine than L-glutamate in a (Ca2+)-dependent manner, leading to activation of adjacent NMDAR receptors and therefore regulates the heterosynaptic long-term depression and metaplasticity during initial memory acquisition. Releases the 4-aminobutanoate neurotransmitter in a (Ca2+)-dependent manner, and participates in its tonic release from cerebellar glial cells. |
| Protein Name | Bestrophin-1Tu15bVitelliform Macular Dystrophy Protein 2 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneLocalized At The Surface Membrane Of Microdomains Adjacent To Glutamatergic Synapses |
| Alternative Antibody Names | Anti-Bestrophin-1 antibodyAnti-Tu15b antibodyAnti-Vitelliform Macular Dystrophy Protein 2 antibodyAnti-BEST1 antibodyAnti-VMD2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
