| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BCR |
| Gene ID: | 613 |
| Uniprot ID: | BCR_HUMAN |
| Immunogen Region: | 1-300 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BCR (NP_004318.3). |
| Immunogen Sequence: | MVDPVGFAEAWKAQFPDSEP PRMELRSVGDIEQELERCKA SIRRLEQEVNQERFRMIYLQ TLLAKEKKSYDRQRWGFRRA AQAPDGASEPRASASRPQPA PADGADPPPAEEPEARPDGE GSPGKARPGTARRPGAAASG ERDDRGPPASVAALRSNFER IRKGHGQPGADAEKPFYVNV EFHHERGLVKVNDKEVSDRI SSLGSQAMQMERKKSQHGAG SSVGDASRPPYRGRSSESS |
| Post Translational Modifications | Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2. |
| Function | Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. The amino terminus contains an intrinsic kinase activity. Functions as an important negative regulator of neuronal RAC1 activity. Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity. Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation. |
| Protein Name | Breakpoint Cluster Region Protein Renal Carcinoma Antigen Ny-Ren-26 |
| Database Links | Reactome: R-HSA-1839117 Reactome: R-HSA-5655302 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013423 |
| Cellular Localisation | Postsynaptic Density Cell Projection Dendritic Spine Axon Synapse |
| Alternative Antibody Names | Anti-Breakpoint Cluster Region Protein antibody Anti-Renal Carcinoma Antigen Ny-Ren-26 antibody Anti-BCR antibody Anti-BCR1 antibody Anti-D22S11 antibody |
Information sourced from Uniprot.org