Anti-BCL2L11 antibody (1-50 aa) (STJ91855)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human BIM at the amino acid range 1-50
General Information
| Short Description | Rabbit polyclonal anti-Bcl-2-like protein 11 (1-50 aa) for use in WB, IHC, IF and ELISA in Human, Mouse, Rat and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCL2L11 |
| Gene ID | 10018 |
| Uniprot ID | B2L11_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human BIM at the amino acid range 1-50 |
| Immunogen Region | 1-50 aa |
| Specificity | Bim Polyclonal Antibody detects endogenous levels of Bim protein. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation. Deubiquitination catalyzed by USP27X stabilizes the protein. Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein. |
| Function | Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. |
| Protein Name | Bcl-2-Like Protein 11Bcl2-L-11Bcl2-Interacting Mediator Of Cell Death |
| Database Links | Reactome: R-HSA-111446Reactome: R-HSA-111453Reactome: R-HSA-193648Reactome: R-HSA-6802952Reactome: R-HSA-8862803Reactome: R-HSA-8952158Reactome: R-HSA-9607240Reactome: R-HSA-9614657 |
| Cellular Localisation | Endomembrane SystemPeripheral Membrane ProteinAssociated With Intracytoplasmic MembranesIsoform Bimel: MitochondrionTranslocates From Microtubules To Mitochondria On Loss Of Cell AdherenceIsoform Biml: MitochondrionIsoform Bims: MitochondrionIsoform Bim-Alpha1: Mitochondrion |
| Alternative Antibody Names | Anti-Bcl-2-Like Protein 11 antibodyAnti-Bcl2-L-11 antibodyAnti-Bcl2-Interacting Mediator Of Cell Death antibodyAnti-BCL2L11 antibodyAnti-BIM antibody |
Information sourced from Uniprot.org