• Immunofluorescence analysis of C6 cells using Bcl-XL Rabbit polyclonal antibody (STJ22777) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Bcl-XL Rabbit polyclonal antibody (STJ22777) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Bcl-XL antibody (STJ22777) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BCL2L1 antibody (1-80) (STJ22777)

SKU:
STJ22777

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BCL2L1 (1-80) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCL2L1
Gene ID: 598
Uniprot ID: B2CL1_HUMAN
Immunogen Region: 1-80
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human Bcl-XL (NP_001304848.1).
Immunogen Sequence: MSQSNRELVVDFLSYKLSQK GYSWSQFSDVEENRTEAPEG TESEMETPSAINGNPSWHLA DSPAVNGATGHSSSLDAREV
Tissue Specificity Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Post Translational Modifications Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. Ubiquitinated by RNF183 during prolonged ER stress, leading to degradation by the proteosome.
Function Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Isoform Bcl-X(S) promotes apoptosis.
Protein Name Bcl-2-Like Protein 1
Bcl2-L-1
Apoptosis Regulator Bcl-X
Database Links Reactome: R-HSA-111453
Reactome: R-HSA-6785807
Reactome: R-HSA-844455
Reactome: R-HSA-9648002
Reactome: R-HSA-9692913
Reactome: R-HSA-9702518
Cellular Localisation Isoform Bcl-X(L): Mitochondrion Inner Membrane
Mitochondrion Outer Membrane
Mitochondrion Matrix
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Cytoplasm
Cytosol
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus Membrane
Single-Pass Membrane Protein
Cytoplasmic Side
After Neuronal Stimulation
Translocates From Cytosol To Synaptic Vesicle And Mitochondrion Membrane In A Calmodulin-Dependent Manner
Localizes To The Centrosome When Phosphorylated At Ser-49
Alternative Antibody Names Anti-Bcl-2-Like Protein 1 antibody
Anti-Bcl2-L-1 antibody
Anti-Apoptosis Regulator Bcl-X antibody
Anti-BCL2L1 antibody
Anti-BCL2L antibody
Anti-BCLX antibody

Information sourced from Uniprot.org

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