Anti-BCKDK antibody (11-60 aa) (STJ91832)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human BCKD at the amino acid range 11-60
General Information
| Short Description | Rabbit polyclonal anti-3-methyl-2-oxobutanoate dehydrogenase kinase, mitochondrial (11-60 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCKDK |
| Gene ID | 10295 |
| Uniprot ID | BCKD_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human BCKD at the amino acid range 11-60 |
| Immunogen Region | 11-60 aa |
| Specificity | BCKDK Polyclonal Antibody detects endogenous levels of BCKDK protein. |
Additional Info
| Post Translational Modifications | Autophosphorylated. |
| Function | Serine/threonine-protein kinase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle. Phosphorylates and inactivates mitochondrial BCKDH complex a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Associates with the E2 component of BCKDH complex and phosphorylates BCKDHA on Ser-337, leading to conformational changes that interrupt substrate channeling between E1 and E2 and inactivates the BCKDH complex. Phosphorylates ACLY on Ser-455 in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and glucogenesis, respectively. Recognizes phosphosites having SxxE/D canonical motif. |
| Protein Name | Branched-Chain Alpha-Ketoacid Dehydrogenase KinaseBckdh KinaseBckdhkinBdk3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial |
| Database Links | Reactome: R-HSA-70895Reactome: R-HSA-9912481 |
| Cellular Localisation | Mitochondrion MatrixDetected In The Cytosolic Compartment Of Liver Cells |
| Alternative Antibody Names | Anti-Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase antibodyAnti-Bckdh Kinase antibodyAnti-Bckdhkin antibodyAnti-Bdk antibodyAnti-3-Methyl-2-Oxobutanoate Dehydrogenase Lipoamide Kinase - Mitochondrial antibodyAnti-BCKDK antibody |
Information sourced from Uniprot.org