• Immunofluorescence analysis of NIH-3T3 cells using BCAT2 Rabbit polyclonal antibody (STJ29562) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of BCAT2 in paraffin-embedded mouse kidney using BCAT2 Rabbit polyclonal antibody (STJ29562) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BCAT2 in paraffin-embedded rat brain using BCAT2 Rabbit polyclonal antibody (STJ29562) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BCAT2 Rabbit polyclonal antibody (STJ29562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using BCAT2 Rabbit polyclonal antibody (STJ29562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BCAT2 antibody (28-392) (STJ29562)

SKU:
STJ29562

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BCAT2 (28-392) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCAT2
Gene ID: 587
Uniprot ID: BCAT2_HUMAN
Immunogen Region: 28-392
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-392 of human BCAT2 (NP_001181.2).
Immunogen Sequence: ASSSFKAADLQLEMTQKPHK KPGPGEPLVFGKTFTDHMLM VEWNDKGWGQPRIQPFQNLT LHPASSSLHYSLQLFEGMKA FKGKDQQVRLFRPWLNMDRM LRSAMRLCLPSFDKLELLEC IRRLIEVDKDWVPDAAGTSL YVRPVLIGNEPSLGVSQPTR ALLFVILCPVGAYFPGGSVT PVSLLADPAFIRAWVGGVGN YKLGGNYGPTVLVQQEALKR GCEQVLWLYGPDHQLTEVG
Tissue Specificity Ubiquitous.
Function Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
Protein Name Branched-Chain-Amino-Acid Aminotransferase - Mitochondrial
Bcat(M
Placental Protein 18
Pp18
Database Links Reactome: R-HSA-70895
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Branched-Chain-Amino-Acid Aminotransferase - Mitochondrial antibody
Anti-Bcat(M antibody
Anti-Placental Protein 18 antibody
Anti-Pp18 antibody
Anti-BCAT2 antibody
Anti-BCATM antibody
Anti-BCT2 antibody
Anti-ECA40 antibody

Information sourced from Uniprot.org

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