Anti-BCAS3 antibody (674-913) (STJ29413)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BCAS3 (674-913) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCAS3 |
| Gene ID | 54828 |
| Uniprot ID | BCAS3_HUMAN |
| Immunogen Region | 674-913 |
| Immunogen Sequence | LAGLVPPGSPGPITRHGSYD SLASDHSGQEDEEWLSQVEI VTHTGPHRRLWMGPQFQFKT IHPSGQTTVISSSSSVLQSH GPSDTPQPLLDFDTDDLDLN SLRIQPVRSDPVSMPGSSRP VSDRRGVSTVIDAASGTFDR SVTLLEVCGSWPEGFGLRHM SSMEHTEEGLRERLADAMAE SPSRDVVGSGTELQREGSIE TLSNSSGSTSGSIPRNFDGY RSPLPTNESQPLSLFPTGF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 674-913 of human BCAS3 (NP_060149.3). |
Additional Info
| Tissue Specificity | Expressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Expressed in undifferentiated ES cells. Expressed in blood islands and nascent blood vessels derived from differentiated ES cells into embryoid bodies (BD). Expressed in endothelial cells. Not detected in brain. Expressed in brain tumors (at protein level). Expressed in brain. Highly expressed in breast cancers and in glioma cell lines. |
| Function | Plays a role in angiogenesis. Participates in the regulation of cell polarity and directional endothelial cell migration by mediating both the activation and recruitment of CDC42 and the reorganization of the actin cytoskeleton at the cell leading edge. Promotes filipodia formation. Functions synergistically with PELP1 as a transcriptional coactivator of estrogen receptor-responsive genes. Stimulates histone acetyltransferase activity. Binds to chromatin. Plays a regulatory role in autophagic activity. In complex with PHAF1, associates with the preautophagosomal structure during both non-selective and selective autophagy. Probably binds phosphatidylinositol 3-phosphate (PtdIns3P) which would mediate the recruitment preautophagosomal structures. |
| Protein Name | Bcas3 Microtubule Associated Cell Migration FactorBreast Carcinoma-Amplified Sequence 3Gaob1 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytoskeletonPreautophagosomal StructureLocalizes In The Cytoplasm In Stationary CellsTranslocates From The Cytoplasm To The Leading Edge In Motile CellsColocalizes With Microtubules And Intermediate Filaments In Both Stationary And Motile CellsAssociates With ChromatinRecruited To Estrogen Receptor-Induced Promoters In A Pelp1-Dependent MannerThe Bcas3:Phaf1 Complex Is Recruited To The Preautophagosomal Structures Adjacent To The Damaged Mitochondria Upon Mitophagy In A Prkn-Pink1 Dependent Manner |
| Alternative Antibody Names | Anti-Bcas3 Microtubule Associated Cell Migration Factor antibodyAnti-Breast Carcinoma-Amplified Sequence 3 antibodyAnti-Gaob1 antibodyAnti-BCAS3 antibody |
Information sourced from Uniprot.org