| Host: | Mouse |
| Applications: | WB/IF |
| Reactivity: | Human/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Apoptosis regulator BAX is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 2B12-G6-H6 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2a |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000IF ICC 1:100-500 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BAX |
| Gene ID: | 581 |
| Uniprot ID: | BAX_HUMAN |
| Specificity: | This antibody detects endogenous levels of Bax and does not cross-react with related proteins. |
| Immunogen: | Purified recombinant human Bax protein fragments expressed in E.coli |
| Post Translational Modifications | Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12. |
| Function | Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
| Protein Name | Apoptosis Regulator BaxBcl-2-Like Protein 4Bcl2-L-4 |
| Database Links | Reactome: R-HSA-111457Reactome: R-HSA-114294Reactome: R-HSA-5620971Reactome: R-HSA-6803204Reactome: R-HSA-6804114Reactome: R-HSA-8878166Reactome: R-HSA-9603505 |
| Cellular Localisation | Isoform Alpha: Mitochondrion Outer MembraneSingle-Pass Membrane ProteinCytoplasmNucleusColocalizes With 14-3-3 Proteins In The CytoplasmUnder Stress ConditionsUndergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion MembraneUpon Sendai Virus InfectionRecruited To The Mitochondrion Through Interaction With Irf3Isoform Beta: CytoplasmIsoform Gamma: CytoplasmIsoform Delta: Cytoplasm |
| Alternative Antibody Names | Anti-Apoptosis Regulator Bax antibodyAnti-Bcl-2-Like Protein 4 antibodyAnti-Bcl2-L-4 antibodyAnti-BAX antibodyAnti-BCL2L4 antibody |
Information sourced from Uniprot.org
