| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-BAG5 (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 55+48kDa bands observed in nuclear lysates of cell line HeLa (calculated MW of 56.0kDa according to NP_001015049.1 and of 51.2kDa according to NP_004864.1; NP_001015048.1). Reco |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | BAG5 |
| Gene ID: | 9529 |
| Uniprot ID: | BAG5_HUMAN |
| Immunogen Region: | N-Term |
| Accession Number: | NP_001015049.1; NP_004864.1; NP_001015048.1 |
| Specificity: | Variants (NP_004864.1; NP_001015048.1) encode the same isoform. |
| Immunogen Sequence: | DMGNQHPSISRLQ |
| Function | Co-chaperone for HSP/HSP70 proteins. It functions as a nucleotide-exchange factor promoting the release of ADP from HSP70, thereby activating HSP70-mediated protein refolding. Has an essential role in maintaining proteostasis at junctional membrane complexes (JMC), where it may function as a scaffold between the HSPA8 chaperone and JMC proteins enabling correct, HSPA8-dependent JMC protein folding. Inhibits both auto-ubiquitination of PRKN and ubiquitination of target proteins by PRKN. |
| Protein Name | Bag Family Molecular Chaperone Regulator 5Bag-5Bcl-2-Associated Athanogene 5 |
| Database Links | Reactome: R-HSA-3371453 |
| Cellular Localisation | In CardiomyocytesLocalized At Specialized Membrane Contact Sites Between T-Tubules And The Sarcoplasmic ReticulumKnown As Junctional Membrane Complexes |
| Alternative Antibody Names | Anti-Bag Family Molecular Chaperone Regulator 5 antibodyAnti-Bag-5 antibodyAnti-Bcl-2-Associated Athanogene 5 antibodyAnti-BAG5 antibodyAnti-KIAA0873 antibody |
Information sourced from Uniprot.org