| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-BAG2 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 25kDa band observed in Human HeLa lysates (calculated MW of 23.7kDa according to NP_004273.1). Recommended concentration: 0.1-0.3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | BAG2 |
| Gene ID: | 9532 |
| Uniprot ID: | BAG2_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_004273.1 |
| Immunogen Sequence: | CSKTLQQNAESRFN |
| Protein Name | Bag Family Molecular Chaperone Regulator 2Bag-2Bcl-2-Associated Athanogene 2 |
| Database Links | Reactome: R-HSA-3371453 |
| Alternative Antibody Names | Anti-Bag Family Molecular Chaperone Regulator 2 antibodyAnti-Bag-2 antibodyAnti-Bcl-2-Associated Athanogene 2 antibodyAnti-BAG2 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and BAG2 knockout (KO) HeLa cells, using [KO Validated] BAG2 Rabbit polyclonal antibody (STJ11100821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89757/358233/STJ11100821_1__05366.1713122564.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BAG2 knockout (KO) HeLa cells, using [KO Validated] BAG2 Rabbit polyclonal antibody (STJ11100821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
