![Western blot analysis of lysates from wild type (WT) and BAG2 knockout (KO) HeLa cells, using [KO Validated] BAG2 Rabbit polyclonal antibody (STJ11100821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89757/358233/STJ11100821_1__05366.1713122564.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BAG2 knockout (KO) HeLa cells, using [KO Validated] BAG2 Rabbit polyclonal antibody (STJ11100821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and BAG2 knockout (KO) HeLa cells, using [KO Validated] BAG2 Rabbit polyclonal antibody (STJ11100821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89757/358233/STJ11100821_1__05366.1713122564.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BAG2 knockout (KO) HeLa cells, using [KO Validated] BAG2 Rabbit polyclonal antibody (STJ11100821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BAG2 |
| Gene ID: | 9532 |
| Uniprot ID: | BAG2_HUMAN |
| Immunogen Region: | 1-211 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human BAG2 (NP_004273.1). |
| Immunogen Sequence: | MAQAKINAKANEGRFCRSSS MADRSSRLLESLDQLELRVE ALREAATAVEQEKEILLEMI HSIQNSQDMRQISDGEREEL NLTANRLMGRTLTVEVSVET IRNPQQQESLKHATRIIDEV VNKFLDDLGNAKSHLMSLYS ACSSEVPHGPVDQKFQSIVI GCALEDQKKIKRRLETLLRN IENSDKAIKLLEHSKGAGSK TLQQNAESRFN |
| Function | Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. |
| Protein Name | Bag Family Molecular Chaperone Regulator 2Bag-2Bcl-2-Associated Athanogene 2 |
| Database Links | Reactome: R-HSA-3371453 |
| Alternative Antibody Names | Anti-Bag Family Molecular Chaperone Regulator 2 antibodyAnti-Bag-2 antibodyAnti-Bcl-2-Associated Athanogene 2 antibodyAnti-BAG2 antibody |
Information sourced from Uniprot.org