• Western blot analysis of lysates from wild type (WT) and BAG2 knockout (KO) HeLa cells, using [KO Validated] BAG2 Rabbit polyclonal antibody (STJ11100821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BAG2 antibody (1-211) (STJ11100821)

SKU:
STJ11100821

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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAG2
Gene ID: 9532
Uniprot ID: BAG2_HUMAN
Immunogen Region: 1-211
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human BAG2 (NP_004273.1).
Immunogen Sequence: MAQAKINAKANEGRFCRSSS MADRSSRLLESLDQLELRVE ALREAATAVEQEKEILLEMI HSIQNSQDMRQISDGEREEL NLTANRLMGRTLTVEVSVET IRNPQQQESLKHATRIIDEV VNKFLDDLGNAKSHLMSLYS ACSSEVPHGPVDQKFQSIVI GCALEDQKKIKRRLETLLRN IENSDKAIKLLEHSKGAGSK TLQQNAESRFN
Function Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.
Protein Name Bag Family Molecular Chaperone Regulator 2
Bag-2
Bcl-2-Associated Athanogene 2
Database Links Reactome: R-HSA-3371453
Alternative Antibody Names Anti-Bag Family Molecular Chaperone Regulator 2 antibody
Anti-Bag-2 antibody
Anti-Bcl-2-Associated Athanogene 2 antibody
Anti-BAG2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance